Time-resolved stimulated Raman scattering microscopy for robust quantitative chemical measurements in tissue

Terumasa Ito; Miyako Iritani; Fumiaki Matsuoka; Kazuhiko Misawa

doi:10.1117/12.2582564

5 March 2021 Time-resolved stimulated Raman scattering microscopy for robust quantitative chemical measurements in tissue

Terumasa Ito, Miyako Iritani, Fumiaki Matsuoka, Kazuhiko Misawa

Author Affiliations +

Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 116560G (2021) https://doi.org/10.1117/12.2582564
Event: SPIE BiOS, 2021, Online Only

Abstract

Quantitative measurements of a small amount of chemicals in label-free tissue imaging by conventional SRS microscopy remain challenging because of background signals. We present a time-resolved, phase-modulated (PM) SRS microscopy method with increased signal contrast. In addition to removing background signals generated via amplitude modulation, PM-SRS can reduce intrinsic tissue background signals by temporally separating the excitation and detection processes. Furthermore, polarization artifacts in tissues can also be removed by rapidly modulating the relative delay between the pump and probe pulses. This new technology enables robust quantitative measurements in tissue and extends the potential of SRS imaging in biomedical applications.

Conference Presentation

Citation Download Citation

Terumasa Ito, Miyako Iritani, Fumiaki Matsuoka, and Kazuhiko Misawa "Time-resolved stimulated Raman scattering microscopy for robust quantitative chemical measurements in tissue", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116560G (5 March 2021); https://doi.org/10.1117/12.2582564

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available