Broadband stimulated Raman scattering microscopy at >1 frame/s with a multi-channel integrated-circuit differential lock-in amplifier

Alejandro De la Cadena; Andrea Ragni; Giuseppe Sciortino; Federico Vernuccio; Carlo Valensise; Marco Sampietro; Giorgio Ferrari; Giulio Cerullo; Dario Polli

doi:10.1117/12.2577680

5 March 2021 Broadband stimulated Raman scattering microscopy at >1 frame/s with a multi-channel integrated-circuit differential lock-in amplifier

Alejandro De la Cadena, Andrea Ragni, Giuseppe Sciortino, Federico Vernuccio, Carlo Valensise, Marco Sampietro, Giorgio Ferrari, Giulio Cerullo, Dario Polli

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Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 116560V (2021) https://doi.org/10.1117/12.2577680
Event: SPIE BiOS, 2021, Online Only

Abstract

Stimulated Raman scattering (SRS) microscopy is a powerful technique for label-free identification of molecules based on their intrinsic vibrational spectrum. We present a novel approach to broadband SRS microscopy based on a recently developed balanced low-noise integrated-circuit multichannel lock-in amplifier with 10-μs integration time and 32 channels working in parallel, enabling multiplex SRS detection at speed faster than one hyperspectral frame per second. The system is powered by a narrowband Stokes pulse at 1040 nm and a broadband (approx. 500 cm^-1) pump pulse generated by a home-built low-noise optical parametric oscillator with up to 100-mW average power, covering the whole C-H stretching band. We measure stimulated Raman loss on the broadband pump pulse employing an in-line balanced detection approach to suppress the laser fluctuations and achieve close to shot-noise-limited sensitivity. We will show the system performances in high-resolution cell imaging.

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Alejandro De la Cadena, Andrea Ragni, Giuseppe Sciortino, Federico Vernuccio, Carlo Valensise, Marco Sampietro, Giorgio Ferrari, Giulio Cerullo, and Dario Polli "Broadband stimulated Raman scattering microscopy at >1 frame/s with a multi-channel integrated-circuit differential lock-in amplifier", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116560V (5 March 2021); https://doi.org/10.1117/12.2577680

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KEYWORDS

Microscopy

Optical amplifiers

Raman scattering

Modulation

Molecules

Optical parametric oscillators

Pulsed laser operation

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