Correction of time-resolved SPAD array measurements for accurate single-photon time-resolved biological imaging

Jakub Nedbal; Francesco Mattioli Della Rocca; Richard Walker; Robert K. Henderson; Klaus Suhling

doi:10.1117/12.2587755

12 April 2021 Correction of time-resolved SPAD array measurements for accurate single-photon time-resolved biological imaging

Jakub Nedbal, Francesco Mattioli Della Rocca, Richard Walker, Robert K. Henderson, Klaus Suhling

Author Affiliations +

Proceedings Volume 11721, Advanced Photon Counting Techniques XV; 117210T (2021) https://doi.org/10.1117/12.2587755
Event: SPIE Defense + Commercial Sensing, 2021, Online Only

Abstract

A 128x192 SPAD array (QuantiCam) with an on-chip time-to-digital converter in each pixel is used as a camera in a single-photon time-resolved fluorescence microscope. The SPAD array introduces systematic nonlinearities and timing offset to the measured photon arrival times. This limits the fidelity of the experimental results. A Monte-Carlo algorithm was developed to transform the raw photon time-stamp stream coming from the SPAD array into a corrected virtual “photon” time-stamp stream devoid of the systematic measurement errors. This data is compatible with existing downstream data processing pipelines used in time-correlated single-photon counting. We discuss the calibration measurement, the algorithm, their performance and application to live fluorescence lifetime imaging of photosynthetic organisms.

Conference Presentation

Citation Download Citation

Jakub Nedbal, Francesco Mattioli Della Rocca, Richard Walker, Robert K. Henderson, and Klaus Suhling "Correction of time-resolved SPAD array measurements for accurate single-photon time-resolved biological imaging", Proc. SPIE 11721, Advanced Photon Counting Techniques XV, 117210T (12 April 2021); https://doi.org/10.1117/12.2587755

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