PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
We present a 3-D non-invasive OCT-based tissue dynamics imaging method to evaluate the tumor spheroid drug response. Our method depends on newly developed 3-D scanning protocol, which acquires the volumetric tomography in 52.4 s. The scanning protocol repeats raster scanning 32 times at each location in the tissue in 6.55 s. The tissue sub-cellular motion/viability is quantified by analyzing the OCT time sequence using our developed algorithms including “logarithmic intensity variance algorithm (LIV)” and “late OCT correlation decay speed (OCDSl)”. The capability of our method has been investigated by evaluating the response of the human originated breast cancer (MCF-7) and colon cancer (HT-29) spheroids to anti-cancer drugs. The tissue viability alterations induced by the drug applications have been successfully visualized and quantified.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.