All-reflective multi-modal nonlinear microscopy with minimal alignment

Benjamin Cromey; Yukun Qin; Orkhongua Batjargal; Joshua Magnus; Sean Crystal; Khanh Kieu

doi:10.1117/12.2608010

2 March 2022 All-reflective multi-modal nonlinear microscopy with minimal alignment

Benjamin Cromey, Yukun Qin, Orkhongua Batjargal, Joshua Magnus, Sean Crystal, Khanh Kieu

Proceedings Volume 11952, Multimodal Biomedical Imaging XVII; 1195207 (2022) https://doi.org/10.1117/12.2608010
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

The design of an all-reflective laser-scanning microscope capable of imaging samples three-dimensionally with multiple modes of nonlinear imaging at high resolution and with a large field of view is described in this presentation. The all-reflective design is based on off-axis parabolic mirror sections, and was designed to require minimal alignment. An all fiber alignment-free femtosecond laser with dual output is used to enable simultaneous multiphoton and stimulated Raman imaging processes, creating a powerful but hassle-free system. This paper will describe the design, performance, and imaging outputs of the system.

Citation Download Citation

Benjamin Cromey, Yukun Qin, Orkhongua Batjargal, Joshua Magnus, Sean Crystal, and Khanh Kieu "All-reflective multi-modal nonlinear microscopy with minimal alignment", Proc. SPIE 11952, Multimodal Biomedical Imaging XVII, 1195207 (2 March 2022); https://doi.org/10.1117/12.2608010

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