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3 March 2022 Nanospeckle illumination microscopy via randomly localized near-field speckle by nanocomposite islands
Hajun Yoo, Hongki Lee, Woo Joong Rhee, Gwiyeong Moon, Changhun Lee, Jeon-Soo Shin, Donghyun Kim
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Proceedings Volume 11976, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIX; 1197603 (2022) https://doi.org/10.1117/12.2607865
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Beyond structured illumination microscopy (SIM) which uses diffraction-limited light illumination, specially designed nanostructures such as metallic nanoantenna arrays generating localized surface plasmon have been developed to expand the frequency information without increasing photon energy. In this study, disordered temperature-annealed nanocomposite islands were used to create random distribution of nanospeckles because nanoisland substrates can be mass-produced in a large observation area by thin film deposition and annealing process. In our nanospeckle illumination microscopy (NanoSIM) system, azimuthal scanning illumination (ASI) on nanoislands creates a randomly localized nearfield distribution that induces an arbitrary number of fluorescence images. By the difficulty of obtaining structured illumination patterns of random nanostructures, images were reconstructed using a modified blind-SIM algorithm which fits well with the ASI system. A 100 nm fluorescent nanobead experiment confirms that NanoSIM provides resolution enhancement of spatial information in good agreement with the results obtained from AFM images. We emphasize that using random nanospeckles of disordered nanocomposite islands can provide highly accessible super-resolution. The results can be applied to imaging and sensing techniques, such as switching-based multi-channel microscopy.
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Hajun Yoo, Hongki Lee, Woo Joong Rhee, Gwiyeong Moon, Changhun Lee, Jeon-Soo Shin, and Donghyun Kim "Nanospeckle illumination microscopy via randomly localized near-field speckle by nanocomposite islands", Proc. SPIE 11976, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIX, 1197603 (3 March 2022); https://doi.org/10.1117/12.2607865
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KEYWORDS
Near field
Microscopy
Speckle
Super resolution
Nanocomposites
Reconstruction algorithms
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