Investigation of ground state interaction through fluorescence decay surfaces of excited state reactions

Marcel Ameloot; Noel Boens

doi:10.1117/12.17741

1 May 1990 Investigation of ground state interaction through fluorescence decay surfaces of excited state reactions

Marcel Ameloot, Noel Boens

Proceedings Volume 1204, Time-Resolved Laser Spectroscopy in Biochemistry II; (1990) https://doi.org/10.1117/12.17741
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

fluorescence decay surfaces of excited state reactions can be globally analyzed directly in terms of reaction rates and species associated spectra [Beechem et al., Chem. Phys. Letters 120 (1985) 466]. The identifiability of two-state excited state reactions has been investigated for properly normalized decay curves assuming that the ratio of the ground state absorbances is known [Ameloot et al, Chem. Phys. Letters 129 [1986] 21 1]. It is demonstrated in this paper that the condition of proper normalization is not always required. In addition, it is shown that the ratio of the absorbances of the species in the ground state can be obtained from fluorescence decay surfaces. The required experimental design is indicated.

Citation Download Citation

Marcel Ameloot and Noel Boens "Investigation of ground state interaction through fluorescence decay surfaces of excited state reactions", Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); https://doi.org/10.1117/12.17741

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available