Two-photon electronic spectra of nucleotides

Scott Williams; Patrik R. Callis

doi:10.1117/12.17699

1 May 1990 Two-photon electronic spectra of nucleotides

Scott Williams, Patrik R. Callis

Proceedings Volume 1204, Time-Resolved Laser Spectroscopy in Biochemistry II; (1990) https://doi.org/10.1117/12.17699
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

Polarized two-photon fluorescence excitation spectra were obtained for the nucleotides TMP, CMP, GMP, and AMP in neutral aqueous room temperature solution (0.05M). The wavelength range was 400- 600 nm, corresponding to states reached with 200-300 nm single UV photons. INDO/S-CI predictions were carried out for the corresponding methyl bases, and were somewhat useful for the 260 nm bands with certain parameterizations. Theory failed to find the strong two-photon absorption seen below 450 nm, which may be of Rydberg origin.

Citation Download Citation

Scott Williams and Patrik R. Callis "Two-photon electronic spectra of nucleotides", Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); https://doi.org/10.1117/12.17699

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