High-speed stroboscopic multispectral imaging of fluorescent probes in living cells

Fernando J. Delaville; Richard A. Tuft; Fredric S. Fay

doi:10.1117/12.17782

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1 August 1990 High-speed stroboscopic multispectral imaging of fluorescent probes in living cells

Fernando J. Delaville, Richard A. Tuft, Fredric S. Fay

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Proceedings Volume 1205, Bioimaging and Two-Dimensional Spectroscopy; (1990) https://doi.org/10.1117/12.17782
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

Studies using fluorescent probes to determine the distribution of physiologically important ions and molecules have been the focus of much interest. Some of these probes may be used to measure the actual concentration of chemical species within cells if measurements are made at two or more wavelengths. However, because of the low photon levels of fluorescent emission at the single cell level, current intensified cameras yield useful information at about 1 second time resolution. Many biologically relevant change; in ionic composition in cells take place much more rapidly than this, therefore requiring a faster imaging system to study these processes in a localized manner. A fast digital fluorescence microscope capable of obtaining dual wavelength images at 30 images per second is described. It can record up to six minutes of data while switching excitation wavelength between image frames.

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Fernando J. Delaville, Richard A. Tuft, and Fredric S. Fay "High-speed stroboscopic multispectral imaging of fluorescent probes in living cells", Proc. SPIE 1205, Bioimaging and Two-Dimensional Spectroscopy, (1 August 1990); https://doi.org/10.1117/12.17782

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