Ex vivo mesoscopic assessment of drug delivery and target engagement via lifetime FRET

Shan Gao; Amit Verma; Margarida Barroso; Xavier Intes

doi:10.1117/12.2650632

14 March 2023 Ex vivo mesoscopic assessment of drug delivery and target engagement via lifetime FRET

Shan Gao, Amit Verma, Margarida Barroso, Xavier Intes

Proceedings Volume 12357, Visualizing and Quantifying Drug Distribution in Tissue VII; 123570F (2023) https://doi.org/10.1117/12.2650632
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Time-domain mesoscopic fluorescence molecular tomography (TD-MFMT) provides a unique window for non-invasively monitoring drug delivery efficacy parameters in targeted anticancer research, such as drug-receptor engagement and intra-tumoral heterogeneity. We investigate the potential of TD-MFMT for ex vivo assessment of Trastuzumab-HER2 target engagement and drug distribution in two types of breast and ovarian cancer tumor xenograft models (AU565 and SK-OV-3) via lifetime FRET technique. FRET fraction level and fluorescence drug distribution results reveal that drug accumulation within tumors cannot be automatically associated with targeted cellular drug delivery.

Conference Presentation

Citation Download Citation

Shan Gao, Amit Verma, Margarida Barroso, and Xavier Intes "Ex vivo mesoscopic assessment of drug delivery and target engagement via lifetime FRET", Proc. SPIE 12357, Visualizing and Quantifying Drug Distribution in Tissue VII, 123570F (14 March 2023); https://doi.org/10.1117/12.2650632

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