In vivo two-photon imaging of topically applied care cosmetics and pharmaceuticals in human skin

Aisada König; Karsten König

doi:10.1117/12.2650427

25 April 2023 In vivo two-photon imaging of topically applied care cosmetics and pharmaceuticals in human skin

Aisada König, Karsten König

Proceedings Volume 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; 1238405 (2023) https://doi.org/10.1117/12.2650427
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We applied femtosecond laser multiphoton tomographs, including the novel system MPTcompact with its chiller-free fiber laser positioned inside the measurement head, for multimodal imaging of in vivo human skin of volunteers, that had been exposed to topically applied sunscreens, UV stains, care cosmetics, and pharmaceuticals. Signals are based on two-photon excited and time-resolved fluorescence (FLIM), Second Harmonic Generation (SHG), and confocal reflection. In particular, SHG signals have been detected from silver nanoparticles in mineral sunscreen. Of particular interest was the distribution of the exogenous compounds within the upper epidermis such as the stratum corneum as an efficient skin barrier. The lateral resolution was 300 nm and the time resolution was 250 picoseconds. In addition, the autofluorescence based on NADH and keratin detection was imaged.

Conference Presentation

Citation Download Citation

Aisada König and Karsten König "In vivo two-photon imaging of topically applied care cosmetics and pharmaceuticals in human skin", Proc. SPIE 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, 1238405 (25 April 2023); https://doi.org/10.1117/12.2650427

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