Coherent exciton coupling in fluorescent protein dimers probed by two-photon polarization ratio

Mikhail Drobizhev; Henry Puhl III; Kirk Hines; Steve S. Vogel

doi:10.1117/12.2657457

25 April 2023 Coherent exciton coupling in fluorescent protein dimers probed by two-photon polarization ratio

Mikhail Drobizhev, Henry Puhl III, Kirk Hines, Steve S. Vogel

Proceedings Volume 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; 123840E (2023) https://doi.org/10.1117/12.2657457
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Coherent excitonic coupling in a dimer of a GFP-analogue, Venus-A206, was discovered previously by using circular dichroism and a combination of photon antibunching and FCS. To further elucidate this effect, here we use a new approach based on the measurements of the two-photon polarization ratio (Omega) as a function of excitation wavelength. In a Venus tandem dimer we observe significant changes of Omega across the spectrum, compared to a negative control with only one chromophore. Our model suggests that only a fraction of dimers are coupled excitonically. Their constituent dipoles make an angle of ~40 degree in an oblique arrangement.

Conference Presentation

Citation Download Citation

Mikhail Drobizhev, Henry Puhl III, Kirk Hines, and Steve S. Vogel "Coherent exciton coupling in fluorescent protein dimers probed by two-photon polarization ratio", Proc. SPIE 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, 123840E (25 April 2023); https://doi.org/10.1117/12.2657457

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