Reflective multi-immersion microscope objectives

Fabian F. Voigt; Thomas Naert; Armin Bahl; Soeren Lienkamp; Fritjof Helmchen

doi:10.1117/12.2648095

16 May 2023 Reflective multi-immersion microscope objectives

Fabian F. Voigt, Thomas Naert, Armin Bahl, Soeren Lienkamp, Fritjof Helmchen

Proceedings Volume 12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; 123850B (2023) https://doi.org/10.1117/12.2648095
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We present a novel way of designing multi-immersion microscope objectives inspired by both the mirror-based eyes of scallops and the Schmidt telescope, a wide-field telescope invented in the 1930s. Despite containing only two optical elements and without a correction collar, our design achieves diffraction-limited imaging performance over mm-scale field-of-views in air and in any homogeneous liquid medium. Based on our concept, we built a prototype two-photon microscope objective and demonstrated its versatility by imaging Xenopus tadpoles cleared with BABB (n=1.56) and by imaging the larval Zebrafish brain in vivo in water.

Citation Download Citation

Fabian F. Voigt, Thomas Naert, Armin Bahl, Soeren Lienkamp, and Fritjof Helmchen "Reflective multi-immersion microscope objectives", Proc. SPIE 12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, 123850B (16 May 2023); https://doi.org/10.1117/12.2648095

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