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16 May 2023 Automation of detecting and tracking 3D vesicle transport using dual-focus optics
Seohyun Lee, Hyuno Kim, Hideo Higuchi
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Proceedings Volume 12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; 123850E (2023) https://doi.org/10.1117/12.2649474
Event: SPIE BiOS, 2023, San Francisco, California, United States
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Abstract
Based on a simple ratiometric intensity computation, dual-focus optics have enabled us to track the vesicle transport in a living cell directly from the fluorescence microscopy images. However, because the acquired dual images from this 3D microscopy can be suffered from optical distortion, the calculation result might not accurate enough for the nano-scale. In this paper, we suggest a linear transformation-based image processing method for the accurate detection and tracking of a vesicle in a living cell. In addition, we present a pipeline for reconstructing the 3D trajectory of the vesicle directly from the images.
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Seohyun Lee, Hyuno Kim, and Hideo Higuchi "Automation of detecting and tracking 3D vesicle transport using dual-focus optics", Proc. SPIE 12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, 123850E (16 May 2023); https://doi.org/10.1117/12.2649474
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KEYWORDS
3D image processing
Image processing
3D tracking
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