Imaging strategies in three-dimensional confocal microscopy

Tony Wilson; Simon J. Hewlett

doi:10.1117/12.19562

1 May 1990 Imaging strategies in three-dimensional confocal microscopy

Tony Wilson, Simon J. Hewlett

Proceedings Volume 1245, Biomedical Image Processing; (1990) https://doi.org/10.1117/12.19562
Event: Electronic Imaging: Advanced Devices and Systems, 1990, Santa Clara, CA, United States

Abstract

We discuss various methods of tuning and improving the strength of the optical sectioning property of both brightfield and fluorescent confocal scanning microscopes. We consider techniques based on using different wavelengths, different sized detectors and pupil plane filters. Experimental results are presented.

Citation Download Citation

Tony Wilson and Simon J. Hewlett "Imaging strategies in three-dimensional confocal microscopy", Proc. SPIE 1245, Biomedical Image Processing, (1 May 1990); https://doi.org/10.1117/12.19562

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