Three dimensional (3D) particle tracking technique has gained significant attention in recent years due to its ability to provide accurate and reliable data on the motion of particles, including micro and nanoscale particles. Here, we present a novel technique based on chromatic aberration that achieves 3D tracking using two cameras. Due to chromatic aberration of the lens, the axial position of the particle is mapped onto the lateral position in the image plane at a specific color, allowing us to use a diffraction grating to determine the lateral position on the color spectrum and thus the axial position of the particle. We also perform experiments on a 6.24 μm polystyrene particle in water, and collect data. We finally implement our method in Python and demonstrate that it performs within the tolerable error range and processes images and makes 3D coordinate predictions at a speed of 1.5 kHz.
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