Steroid penetration through different tissue barriers visualized by phase-modulated stimulated Raman scattering microscopy

Miyako Iritani; Terumasa Ito; Kazuhiko Misawa

doi:10.1117/12.2670638

9 August 2023 Steroid penetration through different tissue barriers visualized by phase-modulated stimulated Raman scattering microscopy

Miyako Iritani, Terumasa Ito, Kazuhiko Misawa

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Proceedings Volume 12630, Advances in Microscopic Imaging IV; 1263006 (2023) https://doi.org/10.1117/12.2670638
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

We analyze the tissue penetration of a steroid drug commonly used for the treatment of dermal and eye inflammations using phase-modulated stimulated Raman scattering (PM-SRS) microscopy. Depth-resolved imaging and penetration profile analysis reveal a clear difference in the drug penetration between the skin epithelial and the corneal epithelial tissue models due to the different barrier properties. The results suggest that PM-SRS imaging can be used to visualize the penetration pathway of topical drugs based on the spatial information of the cell and tissue structures, which should contribute to the design of drug functions.

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Miyako Iritani, Terumasa Ito, and Kazuhiko Misawa "Steroid penetration through different tissue barriers visualized by phase-modulated stimulated Raman scattering microscopy", Proc. SPIE 12630, Advances in Microscopic Imaging IV, 1263006 (9 August 2023); https://doi.org/10.1117/12.2670638

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KEYWORDS

Tissues

Microscopy

Raman scattering

Visualization

Skin

Raman spectroscopy

Signal detection

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