Optical interrogation of striatal ensembles during a minimal motor task

Darcy S. Peterka; Ines Rodrigues-Vaz; Vivek R. Athalye; Rui M. Costa

doi:10.1117/12.3012671

13 March 2024 Optical interrogation of striatal ensembles during a minimal motor task

Darcy S. Peterka, Ines Rodrigues-Vaz, Vivek R. Athalye, Rui M. Costa

Proceedings Volume 12829, Optogenetics and Optical Manipulation 2024; 128290F (2024) https://doi.org/10.1117/12.3012671
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The striatum is a critical brain area in the control and initiation of movement. Recently, the activity in striatal spiny projection neurons (SPNs) has also been shown to be different for different gross body movements, suggesting that activity in striatum is movement-specific and can bias which movements animals will perform. However, these larger actions were performed with different body parts, and may reflect somatotopy more than action specificity, and therefore it is unclear how fine the granularity of movement-specificity is. To probe this, we have developed an isometric force task that requires no overt movement, and imaged and analyzed the activity in striatum during learning and execution. From the optically recorded neural activity combined with simultaneous behavior, we trained classifiers that could predict action from activity across days, revealing stable ensembles encoded specific actions. Finally, using targeted two-photon stimulation, we perturbed small sub-populations of these ensembles in closed-loop during behavior, seemingly leading to small, but perceptible, changes in action.

Conference Presentation

Citation Download Citation

Darcy S. Peterka, Ines Rodrigues-Vaz, Vivek R. Athalye, and Rui M. Costa "Optical interrogation of striatal ensembles during a minimal motor task", Proc. SPIE 12829, Optogenetics and Optical Manipulation 2024, 128290F (13 March 2024); https://doi.org/10.1117/12.3012671

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KEYWORDS

Animals

Brain

Education and training

Image analysis

Motion analysis

Neurons

Reflection

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