High-power VECSEL with intracavity UV generation for flow cytometry applications

K. Nechay; R. Perttilä; A. Essaulova; P. Uusimaa

doi:10.1117/12.3002263

12 March 2024 High-power VECSEL with intracavity UV generation for flow cytometry applications

K. Nechay, R. Perttilä, A. Essaulova, P. Uusimaa

Proceedings Volume 12853, High-Speed Biomedical Imaging and Spectroscopy IX; 1285306 (2024) https://doi.org/10.1117/12.3002263
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Flow cytometry is a fundamental and powerful technique in the biomedical sciences which heavily depends on excitation lasers for imaging fluorescent markers to perform cell sorting and analysis. Modern multicolor flow cytometers incorporate several excitation lasers, typically 405 nm, 488 nm, 561 nm, and 640 nm allowing simultaneous cell characterization. Ultraviolet (UV) lasers, namely 355 nm, became indispensable flow cytometry excitation sources with the development of the Brilliant Ultraviolet (BUV) fluorochromes enabling high-dimensional analysis. Primary UV source for BUV excitation is represented by frequency tripled Nd:YVO₄ diode-pumped solid-state lasers (DPSSL) which constitute the single most expensive component of high-end flow cytometers. We demonstrate high-power UV generation from cascade intracavity frequency conversion based on the optically pumped vertically-external-cavity surface-emitting lasers (VECSELs). The active semiconductor gain medium is designed for operation around 1060 nm and is comprised of InGaAs quantum wells. The frequency conversion is implemented in a compact V-shaped cavity with the non-linear lithium triborate (LBO) crystals placed at the waist of the cavity mode in order to enhance conversion efficiency. This relatively novel and cost-effective semiconductor laser platform offers high-power, tunable, low-noise emission with excellent beam quality, important for flow cytometry applications. VECSEL platform can fill the spectral gaps where semiconductor diode laser technology is not available, namely green, and green-yellow spectral regions, and at the same time serving as a cost-effective replacement of DPSSL in the UV and DUV spectral bands.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

K. Nechay, R. Perttilä, A. Essaulova, and P. Uusimaa "High-power VECSEL with intracavity UV generation for flow cytometry applications", Proc. SPIE 12853, High-Speed Biomedical Imaging and Spectroscopy IX, 1285306 (12 March 2024); https://doi.org/10.1117/12.3002263

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