Super-resolution surface imaging of live cells with sub-diffraction-limited plasmonic nanospeckle

Hajun Yoo; Hongki Lee; Woo Joong Rhee; Gwiyeong Moon; Changhun Lee; Jeon-Soo Shin; Donghyun Kim

doi:10.1117/12.3000596

13 March 2024 Super-resolution surface imaging of live cells with sub-diffraction-limited plasmonic nanospeckle

Hajun Yoo, Hongki Lee, Woo Joong Rhee, Gwiyeong Moon, Changhun Lee, Jeon-Soo Shin, Donghyun Kim

Proceedings Volume 12860, Plasmonics in Biology and Medicine XXI; 1286007 (2024) https://doi.org/10.1117/12.3000596
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Nanospeckle Illumination Microscopy (NanoSIM) utilizes plasmonic nanoisland structures to enable super-resolution surface imaging of live cells. By analyzing the intensity fluctuations of plasmonic nanospeckles, we achieved three-fold improved spatial resolution and the ability to identify multiple cellular structures. Experimental results demonstrate the potential of NanoSIM as an effective and versatile tool for investigating dynamic cellular processes within live cell membranes of HeLa cells, providing crucial insights into complex cellular interactions.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Hajun Yoo, Hongki Lee, Woo Joong Rhee, Gwiyeong Moon, Changhun Lee, Jeon-Soo Shin, and Donghyun Kim "Super-resolution surface imaging of live cells with sub-diffraction-limited plasmonic nanospeckle", Proc. SPIE 12860, Plasmonics in Biology and Medicine XXI, 1286007 (13 March 2024); https://doi.org/10.1117/12.3000596

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