Weakly supervised detection of cell activation

Zhilin Zou; Kicheon Park; Jiaxiang Ren; Wensheng Cheng; Sophia Liu; Yingtian Pan; Congwu Du; Haibin Ling

doi:10.1117/12.3005371

2 April 2024 Weakly supervised detection of cell activation

Zhilin Zou, Kicheon Park, Jiaxiang Ren, Wensheng Cheng, Sophia Liu, Yingtian Pan, Congwu Du, Haibin Ling

Author Affiliations +

Proceedings Volume 12930, Medical Imaging 2024: Clinical and Biomedical Imaging; 129301J (2024) https://doi.org/10.1117/12.3005371
Event: SPIE Medical Imaging, 2024, San Diego, California, United States

Conference Poster

Abstract

Traditional stimulation-evoked cortical activity analysis mainly relies on the manual detection of the bright spots in a specific size regarded as active cells. However, there is limited research on the automatic detection of the cortical active cell in optical imaging of in vivo experiments which is much noisy. To address the laborious and hard annotation work, we propose a novel weakly supervised approach for active cell detection along the temporal frames. Compared to prevalent detection methods on common datasets, we formulate cell activation detection as a classification problem. We combine the techniques of clustering and deep neural network with little user indication of the Maximum Intensity Projection (MIP) of the time-lapse optical image sequence to realize the unsupervised classification model. The proposed approach achieves comparable performance on our optical image sequence with instant activation changing at each frame, which marks the cells using the fluorescent indicators. Although much noise is introduced during in vivo imaging, our algorithm is designed to accurately and effectively generate statistics on cell activation without requiring any prior training data preparation. This feature makes it particularly valuable for analyzing cell responses to psychopharmacological stimulation in subsequent analyses.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Zhilin Zou, Kicheon Park, Jiaxiang Ren, Wensheng Cheng, Sophia Liu, Yingtian Pan, Congwu Du, and Haibin Ling "Weakly supervised detection of cell activation", Proc. SPIE 12930, Medical Imaging 2024: Clinical and Biomedical Imaging, 129301J (2 April 2024); https://doi.org/10.1117/12.3005371

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE