A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution

Xiaomei Liu; Jingtan Zhu; Jianyi Xu; Yating Deng; Tingting Yu; Dan Zhu

doi:10.1117/12.3036876

20 November 2024 A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution

Xiaomei Liu, Jingtan Zhu, Jianyi Xu, Yating Deng, Tingting Yu, Dan Zhu

Author Affiliations +

Proceedings Volume 13242, Optics in Health Care and Biomedical Optics XIV; 132422B (2024) https://doi.org/10.1117/12.3036876
Event: SPIE/COS Photonics Asia, 2024, Nantong, Jiangsu, China

Conference Poster

Abstract

Effective labeling of the vascular system is essential for understanding the vascular networks of tissues and organs. Here, we present VALID, a vascular labeling method that visualizes vascular networks through tissue clearing and light-sheet microscopy. VALID converts traditional lipophilic dye solutions into hydrogels by introducing gelatin and inhibits the aggregation of dyes, thus obtaining a complete and uniform vascular labeling pattern with high signal-to-value ratio. VALID enhances the compatibility of lipophilic dyes with solvent-based tissue clearance regimens, which was previously difficult to achieve. Using VALID, we combined lipophile dyes with solvent-based tissue clearing to perform 3D reconstruction of the vascular system within the brain and spinal cord of mice. We also used VALID to visualize and quantify microvascular damage in a mouse model of middle cerebral artery occlusion. VALID offers a simple, cost-effective vascular labeling protocol that will greatly expand the use of lipophilic dyes in the study of cerebrovascular complications.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Xiaomei Liu, Jingtan Zhu, Jianyi Xu, Yating Deng, Tingting Yu, and Dan Zhu "A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution", Proc. SPIE 13242, Optics in Health Care and Biomedical Optics XIV, 132422B (20 November 2024); https://doi.org/10.1117/12.3036876

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