Time-lapse imaging of tumor spheroid drug response by dynamic optical coherence tomography integrated with cell cultivation chamber

Ibrahim Abd El-Sadek; Rion Morishita; Atsuko Furukawa; Masato Iwatsuki; Shuichi Makita; Pradipta Mukherjee; Satoshi Matsusaka; Yoshiaki Yasuno

doi:10.1117/12.3032327

31 December 2024 Time-lapse imaging of tumor spheroid drug response by dynamic optical coherence tomography integrated with cell cultivation chamber

Ibrahim Abd El-Sadek, Rion Morishita, Atsuko Furukawa, Masato Iwatsuki, Shuichi Makita, Pradipta Mukherjee, Satoshi Matsusaka, Yoshiaki Yasuno

Author Affiliations +

Proceedings Volume 13487, Optics and Photonics International Congress 2024; 1348705 (2024) https://doi.org/10.1117/12.3032327
Event: Optics and Photonics International Congress, 2024, Yokohama, Japan

Abstract

We demonstrate longitudinal drug response imaging of tumor spheroids by integrating a spheroid cultivation chamber and the dynamic optical coherence tomography (DOCT) microscope. The cultivation chamber supports the spheroids with 5% of CO₂ and a temperature of 37°C. In contrast to our previous cross-sectional time-course imaging method, this newly integrated system enables longitudinal time-course imaging of a single sample, and hence enables high-time-resolution imaging of a living sample. It successfully revealed the fine temporal and spatial responses of human breast cancer (MCF-7 cell-line) spheroids to paclitaxel (PTX) and doxorubicin hydrochloride (DOX).

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Ibrahim Abd El-Sadek, Rion Morishita, Atsuko Furukawa, Masato Iwatsuki, Shuichi Makita, Pradipta Mukherjee, Satoshi Matsusaka, and Yoshiaki Yasuno "Time-lapse imaging of tumor spheroid drug response by dynamic optical coherence tomography integrated with cell cultivation chamber", Proc. SPIE 13487, Optics and Photonics International Congress 2024, 1348705 (31 December 2024); https://doi.org/10.1117/12.3032327

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