Three-dimensional reconstruction and lateral views in optical microscopy

Tullio Tommasi; Bruno Bianco; Vittorio Murino; Alessandra Oneto; Alberto Diaspro

doi:10.1117/12.24251

1 September 1990 Three-dimensional reconstruction and lateral views in optical microscopy

Tullio Tommasi, Bruno Bianco, Vittorio Murino, Alessandra Oneto, Alberto Diaspro

Proceedings Volume 1360, Visual Communications and Image Processing '90: Fifth in a Series; (1990) https://doi.org/10.1117/12.24251
Event: Visual Communications and Image Processing '90, 1990, Lausanne, Switzerland

Abstract

The three-dimensional properties of an optical microscope are analyzed and a defocusing technique is proposed to recover the spatial distribution of the specimens under investigation. Limitations and real capabilities of the 3D reconstruction are pointed out. As a result, lateral views are obtained by means of operations in the spatial frequency domain. In such a way, it is possible to represent side views of an object within the angular aperture range of the microscope. A theory concerning image formation is discussed and simulations of side view reconstructions are reported.

Citation Download Citation

Tullio Tommasi, Bruno Bianco, Vittorio Murino, Alessandra Oneto, and Alberto Diaspro "Three-dimensional reconstruction and lateral views in optical microscopy", Proc. SPIE 1360, Visual Communications and Image Processing '90: Fifth in a Series, (1 September 1990); https://doi.org/10.1117/12.24251

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