DNA photoproducts formed using high-intensity 532 nm laser radiation

Irene E. Kochevar; Yaron Hefetz; D. A. Dunn; T. F. Deutsch; Lisa A. Buckley; Franz Hillenkamp

doi:10.1117/12.57311

1 May 1991 DNA photoproducts formed using high-intensity 532-nm laser radiation

Irene E. Kochevar, Yaron Hefetz, D. A. Dunn, T. F. Deutsch, Lisa A. Buckley, Franz Hillenkamp

Proceedings Volume 1403, Laser Applications in Life Sciences; (1991) https://doi.org/10.1117/12.57311
Event: Laser Applications in Life Sciences, 1990, Moscow, Russian Federation

Abstract

Cyclobutylpyrimidine dimers were detected in double-stranded plasmid DNA after exposure to 532-nm 28-ps pulses at intensities (1-8 GW/cm2) below the threshold for optical breakdown. Formation of these photoproducts was attributed to simultaneous two-photon absorption by nucleotides in DNA because the rate of dimer formation was dependent on the square of the intensity and because an equivalent number of photons at 514 nm delivered at low intensity did not produce dimers. The average two-photon cross-section at 532 rim for absorption by a nucleotide was found to be 0. 50 ( 0. 02) x l052 cm4sec'' photon* Intensities of 532-rim radiation above the threshold for optical breakdown caused DNA strand breaks by radical and mechanical mechanisms. 1 .

Citation Download Citation

Irene E. Kochevar, Yaron Hefetz, D. A. Dunn, T. F. Deutsch, Lisa A. Buckley, and Franz Hillenkamp "DNA photoproducts formed using high-intensity 532-nm laser radiation", Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); https://doi.org/10.1117/12.57311

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available