Picosecond kinetics and Sn S1 absorption spectra of retinoids and carotenoids

Stanislav L. Bondarev; S. A. Tikhomirov; Sergei M. Bachilo

doi:10.1117/12.57380

1 May 1991 Picosecond kinetics and S_n <-- S₁ absorption spectra of retinoids and carotenoids

Stanislav L. Bondarev, S. A. Tikhomirov, Sergei M. Bachilo

Proceedings Volume 1403, Laser Applications in Life Sciences; (1991) https://doi.org/10.1117/12.57380
Event: Laser Applications in Life Sciences, 1990, Moscow, Russian Federation

Abstract

Light energy absorption, as well as the subsequent photochemical and photophysical processes of cis -+trans isomerisation (vision and bacteriorhodopsin photosynthesis) and energy transfer (photosynthesis in green plants and micro organisms) take place in a pigment-protein complex including polyene chromophors, retinoids and carotenoids. Picosecond and subpicosecond studies of the spectral and kinetic characteristics of these processes are carried out in both complex photoreceptor and photosynthetic ms'2 and model systems with the use of solutions of retinoids and carotenoids.36 The lifetimes of the lower singlet-exited states S (21A; ) ofsome carotenoids in toluene at room temperature have been measured by the method of picosecond photolysis and amount to 8.6± 0.5 for all-trans-fl -carotene1 and 5.2 0.6 PS for canthaxanthin.5 /3 -carotene fluorescence at room temperature is practically absent, its yield being less than iO (Ref. 7). /1 -carotene fluorescence at 77 and 4.2 K in isopentane discovered by us8 is characterized by yields of (4±2) .iO and (8±3) . i0- and lifetimes of(4±2) .iO' and (8±3) .iO' and is due to the transitions from the higher S(1' B) state. The picosecond transient S -S absorption of/I - carotene in different solvents at 293 K is characterized by spectra in the 550-600 nm range.8 For retinoids, there is one work (Ref. 4) which gives the S, +-Si absorption spectrum of the Schiff base (aldimine) of retinal with amaz 465 mn in n-hexane at 290 K. The duration of transient absorption was 21 5 ps, although the fluorescence kinetics measured in this work (Ref. 4) at 298 K were characterized by two-component decay with r1 = 22 and r2 = 265 ps. The transient picosecond absorption spectra for retinal are absent in the literature and the lifetimes of its singlet-excited state at room temperature, measured by absorption and fluorescence, amount to 20±10 Ps in n-hexane3 and 17 Ps in ethanol,'9 respectively.

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Stanislav L. Bondarev, S. A. Tikhomirov, and Sergei M. Bachilo "Picosecond kinetics and S_n <-- S₁ absorption spectra of retinoids and carotenoids", Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); https://doi.org/10.1117/12.57380

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