Molecular tissue mapping by FTIR microspectroscopy

John A. Reffner; Frank M. Wasacz

doi:10.1117/12.56323

1 March 1992 Molecular tissue mapping by FTIR microspectroscopy

John A. Reffner, Frank M. Wasacz

Proceedings Volume 1575, 8th Intl Conf on Fourier Transform Spectroscopy; (1992) https://doi.org/10.1117/12.56323
Event: Eighth International Conference on Fourier Transform Spectroscopy, 1991, Lubeck-Travemunde, Germany

Abstract

Determining the molecular chemical composition of human tissue is important to understanding its biochemistry. Variation in a tissue's biochemistry may result from normal changes in tissue type or from artifacts, which range from foreign materials trapped in tissue to the degeneration products associated with disease. While pathologists use light microscopy to identify abnormal areas of tissue, they can obtain only limited molecular information through staining or fluorescence methods. However, when microscopy and infrared spectroscopy are combined, they produce a powerful technique for probing the chemistry of different areas of tissue samples. In addition, the integration of FT-IR microanalysis with a scanning stage produces compositional maps that directly link molecular structure and morphology.

Citation Download Citation

John A. Reffner and Frank M. Wasacz "Molecular tissue mapping by FTIR microspectroscopy", Proc. SPIE 1575, 8th Intl Conf on Fourier Transform Spectroscopy, (1 March 1992); https://doi.org/10.1117/12.56323

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