Effect of ascorbate on photosensitization of hepatoma cells by hematoporphyrin derivative

Yuqing Chen; Yongzhi Liu; Jian Wang

doi:10.1117/12.137019

5 March 1993 Effect of ascorbate on photosensitization of hepatoma cells by hematoporphyrin derivative

Yuqing Chen, Yongzhi Liu, Jian Wang

Proceedings Volume 1616, International Conference on Photodynamic Therapy and Laser Medicine; (1993) https://doi.org/10.1117/12.137019
Event: International Conference on Photodynamic Therapy and Laser Medicine, 1991, Beijing, China

Abstract

Mouse hepatoma cells, mitochondria, and lysosomes of rat liver cells after incubation with hematoporphyrin derivative (HPD) were treated by ascorbate and exposed to light. The product of membrane lipid peroxidation -- malondialdehyde (MDA) -- was determined. The MDA level increased with the increase of ascorbate content (in a range of certain concentrations) and irradiation time. Thiourea, an inhibitor of hydroxyl free radical, showed an inhibiting effect to the production of MDA in the experimental groups. The MDA level decreased with the increase of thiourea concentration. Ear index, a parameter to evaluate photosensitive reaction of mouse skin by HPD plus light was determined. The value of ear index of mice treated with ascorbate was greater than that of mice without ascorbate treatment. Those results suggest that hydroxyl free radical produced by HPD and ascorbate after light irradiation could oxidize membrane lipids, and showed that ascorbate may enhance porphyrin photosensitization of tumor cells.

Citation Download Citation

Yuqing Chen, Yongzhi Liu, and Jian Wang "Effect of ascorbate on photosensitization of hepatoma cells by hematoporphyrin derivative", Proc. SPIE 1616, International Conference on Photodynamic Therapy and Laser Medicine, (5 March 1993); https://doi.org/10.1117/12.137019

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