Effect of photodynamic therapy on mouse platelets

Chuannong Zhou; Shunji Chi; Jinsheng Deng; Hua Zhang; Junlin Liang; Xian-wen Ha

doi:10.1117/12.136976

5 March 1993 Effect of photodynamic therapy on mouse platelets

Chuannong Zhou, Shunji Chi, Jinsheng Deng, Hua Zhang, Junlin Liang, Xian-wen Ha

Proceedings Volume 1616, International Conference on Photodynamic Therapy and Laser Medicine; (1993) https://doi.org/10.1117/12.136976
Event: International Conference on Photodynamic Therapy and Laser Medicine, 1991, Beijing, China

Abstract

Normal mice received hematoporphyrin derivative (10 mg/kg iv) immediately, 24 or 48 hrs prior to red light irradiation. The blood was collected and the platelet-rich plasma was irradiated by red light (100 J/cm²). The platelets were fixed immediately, 8 or 16 hrs after irradiation, and processed for EM examination. In comparison with those of control mice, the platelets of all experimental mice showed structural changes: 16 hrs after irradiation all platelets were necrotized; 8 hrs after irradiation almost one fourth of the platelets were necrotized and the remaining were considerably damaged; immediately after irradiation a small number of platelets became necrotic and most other platelets were swollen and deformed, often with many cytoplasmic projections and considerable dilatation of the canalicular membrane system. Our findings provided a clear evidence that platelets are highly sensitive to PDT action and can be directly and rapidly injured by PDT even in the absence of vascular endothelial cells. Our results give firm support to the hypothesis that both endothelial cells and platelets may play an important role in the initiation of early vascular damage and microcirculatory alterations induced by PDT in vivo.

Citation Download Citation

Chuannong Zhou, Shunji Chi, Jinsheng Deng, Hua Zhang, Junlin Liang, and Xian-wen Ha "Effect of photodynamic therapy on mouse platelets", Proc. SPIE 1616, International Conference on Photodynamic Therapy and Laser Medicine, (5 March 1993); https://doi.org/10.1117/12.136976

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