Definition of the energy map and its applications to the study of chromophores in proteins

Andras D. Kaposi; V. Logovinsky; Jane M. Vanderkooi

doi:10.1117/12.58275

1 April 1992 Definition of the energy map and its applications to the study of chromophores in proteins

Andras D. Kaposi, V. Logovinsky, Jane M. Vanderkooi

Proceedings Volume 1640, Time-Resolved Laser Spectroscopy in Biochemistry III; (1992) https://doi.org/10.1117/12.58275
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

The porphyrin in metal-free cytochrome c molecules was investigated using fluorescence line narrowing spectroscopy. An energy map is defined to illustrate the environmental effects on the vibronic structure of a chromophore. In the approximation of pure electronic distortion, the population distribution in the energy scale can be characterized by a function. An intuitive definition of this distribution function is determined for the porphyrin in cytochrome c, and the validity of this approximation is experimentally verified. In addition, numerous excited state vibrational levels suggest a method to quantify the relative absorption probabilities for (0,0)yields(1,k) transitions.

Citation Download Citation

Andras D. Kaposi, V. Logovinsky, and Jane M. Vanderkooi "Definition of the energy map and its applications to the study of chromophores in proteins", Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); https://doi.org/10.1117/12.58275

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