Fluorescence decay and depolarization of probes in membranes

Dmitri D. Toptygin; Jaroslava Svobodova; Ivo Konopasek; Ludwig Brand

doi:10.1117/12.58270

1 April 1992 Fluorescence decay and depolarization of probes in membranes

Dmitri D. Toptygin, Jaroslava Svobodova, Ivo Konopasek, Ludwig Brand

Proceedings Volume 1640, Time-Resolved Laser Spectroscopy in Biochemistry III; (1992) https://doi.org/10.1117/12.58270
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

Radiative decay rate depends on the efficiency of coupling between the emission dipole and the electromagnetic field. Therefore, in optically discontinuous and/or anisotropic environments the radiative rate is dependent on the orientation of the emission dipole. Single-bilayer phospholipid membranes are interesting systems for the study of this phenomenon because in these systems the functional dependence of the radiative rate is simple and the effect is stronger than in liquid crystals. The orientational dependence of the radiative rate results in a non-exponential total fluorescence intensity decay. Orientational distribution of the probe in the gel-phase membrane can be determined from the total intensity decay. In the liquid-phase membrane the coupling between decay and rotation leads to a polarized intensity decay behavior entirely different than that in isotropic media. Experimental data are consistent with every theoretical prediction.

Citation Download Citation

Dmitri D. Toptygin, Jaroslava Svobodova, Ivo Konopasek, and Ludwig Brand "Fluorescence decay and depolarization of probes in membranes", Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); https://doi.org/10.1117/12.58270

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