Cold laser technique for cell surgery

Daniel V. Palanker; Shoshanit Ohad; Aaron Lewis; Neri Laufer

doi:10.1117/12.137447

7 August 1992 Cold laser technique for cell surgery

Daniel V. Palanker, Shoshanit Ohad, Aaron Lewis, Neri Laufer

Proceedings Volume 1646, Laser-Tissue Interaction III; (1992) https://doi.org/10.1117/12.137447
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

A new cell surgery technique has been developed to produce well-defined alterations in cells and tissues without detectable heating and/or other structural damage in the surroundings. The technique involves the use of a 193 nm argon fluoride excimer laser which is guided through a glass pipette filled with a positive air pressure. To demonstrate the method holes were drilled in the zona pellucida of mouse oocytes. The diameter of the drilled hole was determined by the pipette tip size, and its depth by an energy emitted per pulse and number of pulses. Scanning electron microscopy of the drilled mouse oocytes showed uniform, round, well circumscribed holes with sharp edges. Oocytes that had their zona pellucida drilled with this new method fertilized in vitro and developed to the blastocyst stage in a rate similar to that of control group. These results demonstrate the non-perturbing nature of this cold laser microsurgical procedure. In addition to the extension of our results for clinical in vitro fertilization purposes, such as enhancement of fertilization and embryo biopsy, there are wide ranging possible uses of our method in fundamental and applied investigations that require sub-micron accuracy in cellular alteration.

Citation Download Citation

Daniel V. Palanker, Shoshanit Ohad, Aaron Lewis, and Neri Laufer "Cold laser technique for cell surgery", Proc. SPIE 1646, Laser-Tissue Interaction III, (7 August 1992); https://doi.org/10.1117/12.137447

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