High-resolution confocal transmission microscope, Part II: determining image position and correcting aberrations

John W. O'Byrne; Carol J. Cogswell

doi:10.1117/12.59580

26 June 1992 High-resolution confocal transmission microscope: Part II - determining image position and correcting aberrations

John W. O'Byrne, Carol J. Cogswell

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Proceedings Volume 1660, Biomedical Image Processing and Three-Dimensional Microscopy; (1992) https://doi.org/10.1117/12.59580
Event: SPIE/IS&T 1992 Symposium on Electronic Imaging: Science and Technology, 1992, San Jose, CA, United States

Abstract

In order to produce high resolution images of complex biological specimens in a confocal transmission microscope, two phenomena must be overcome. Firstly, non-uniform refractive index variations in the specimen cause deflection of the focused image spot in three- dimensional space which presents a problem for the usual confocal approach of imaging onto a fixed pinhole. The second effect is optical aberrations (especially spherical aberration) which arise as a natural function of imaging through the thickness of a refractive specimen. This paper discusses how both effects may be monitored and overcome, while still providing confocal imaging, using a CCD array as the detector

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John W. O'Byrne and Carol J. Cogswell "High-resolution confocal transmission microscope: Part II - determining image position and correcting aberrations", Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); https://doi.org/10.1117/12.59580

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