Shape change analysis of confocal microscope images using variational techniques

Keith A. Bartels; Alan Conrad Bovik; Shanti J. Aggarwal; Kenneth R. Diller

doi:10.1117/12.59589

26 June 1992 Shape change analysis of confocal microscope images using variational techniques

Keith A. Bartels, Alan Conrad Bovik, Shanti J. Aggarwal, Kenneth R. Diller

Proceedings Volume 1660, Biomedical Image Processing and Three-Dimensional Microscopy; (1992) https://doi.org/10.1117/12.59589
Event: SPIE/IS&T 1992 Symposium on Electronic Imaging: Science and Technology, 1992, San Jose, CA, United States

Abstract

A technique for modeling shape changes in microscopic images is described. The technique consists of first segmenting the image to locate the specimen and the parametrizing the specimen in the initial image with an orthogonal material coordinate system. The deformation of the material coordinate system caused by the motion of the specimen is then solved for by minimizing an energy functional which is derived here. Results from both synthetic and real two-dimensional images are presented. The foundations of a three-dimensional implementation are given. A two-dimensional implementation is demonstrated while keeping sufficient generality for an application to three-dimensional dynamic confocal microscope images.

Citation Download Citation

Keith A. Bartels, Alan Conrad Bovik, Shanti J. Aggarwal, and Kenneth R. Diller "Shape change analysis of confocal microscope images using variational techniques", Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); https://doi.org/10.1117/12.59589

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