Preparation of natural phytoplankton communities to preserve spectral fluorescence properties

Susan L. Bower; Rodolfo H. Iturriaga

doi:10.1117/12.140653

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31 December 1992 Preparation of natural phytoplankton communities to preserve spectral fluorescence properties

Susan L. Bower, Rodolfo H. Iturriaga

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Proceedings Volume 1750, Ocean Optics XI; (1992) https://doi.org/10.1117/12.140653
Event: San Diego '92, 1992, San Diego, CA, United States

Abstract

A technique that allows for the preservation of spectral fluorescence properties of phytoplankton cells is presented. Laboratory cultures and field samples were concentrated and embedded within gelatin to preserve the spectral fluorescence properties of phytoplankton cells. Fluorescence excitation spectra (Ex: 400 - 650 nm, Em: 683 nm) obtained from samples prepared according to this method were compared to the spectra obtained from traditional cell suspensions, and by microphotometry. The spectra produced by samples prepared following this method remain spectrally intact if stored at -70 degree(s)C, and allowed to return to room temperature prior to measurement.

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Susan L. Bower and Rodolfo H. Iturriaga "Preparation of natural phytoplankton communities to preserve spectral fluorescence properties", Proc. SPIE 1750, Ocean Optics XI, (31 December 1992); https://doi.org/10.1117/12.140653

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