Time-domain fluorescence methods as applied to pH sensing

Max E. Lippitsch; Sonja Draxler; Marc J. P. Leiner

doi:10.1117/12.143521

30 April 1993 Time-domain fluorescence methods as applied to pH sensing

Max E. Lippitsch, Sonja Draxler, Marc J. P. Leiner

Proceedings Volume 1796, Chemical, Biochemical, and Environmental Fiber Sensors IV; (1993) https://doi.org/10.1117/12.143521
Event: Fibers '92, 1992, Boston, MA, United States

Abstract

Sensors based on luminescence suffer from the fact that during the operating time of the instrument changes in source intensity, light throughput, detector sensitivity, indicator quantum yield, and indicator concentration are inevitable and have to be overcome by extensive referencing and recalibration procedures. Sensors based on luminescence decay time should not suffer from these drawbacks. Decay-time sensing has relied so far on dynamic quenching, which is not well suited for pH measurements. Several other mechanisms are described in this contribution. The conditions necessary for an indicator to be useful in a decay time based pH sensing scheme are clarified and the suitability of this scheme is demonstrated.

Citation Download Citation

Max E. Lippitsch, Sonja Draxler, and Marc J. P. Leiner "Time-domain fluorescence methods as applied to pH sensing", Proc. SPIE 1796, Chemical, Biochemical, and Environmental Fiber Sensors IV, (30 April 1993); https://doi.org/10.1117/12.143521

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