Laser-assisted cell fusion and cytoplast transfer in early mammalian embryos

Annette Clement-Sengewald; Karin Schutze; A. Heinze; G. A. Palma; H. Poesl; G. Brem

doi:10.1117/12.147030

1 July 1993 Laser-assisted cell fusion and cytoplast transfer in early mammalian embryos

Annette Clement-Sengewald, Karin Schutze, A. Heinze, G. A. Palma, H. Poesl, G. Brem

Proceedings Volume 1876, Lasers in Otolaryngology, Dermatology, and Tissue Welding; (1993) https://doi.org/10.1117/12.147030
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

A UV-laser microbeam was successfully used to induce fusion of early embryonic cells. The developmental capacity of the laser-fused cells was examined using in vitro culture methods. Blastomeres within mouse two-cell embryos were fused with 3 - 10 subsequent laser pulses in order to produce tetraploid embryos. Thirty-one percent of the laser treated embryos fused and 10% of those developed to the morula or blastocyst stage. With 1 - 10 successive laser pulses cattle oocytes were fused with cytoplasts. Thirty-six percent of the laser treated cells fused and 10% of those cleaved to the 6- and 8-cell stage. These preliminary results indicate that a UV- laser microbeam combined with an optical tweezers may facilitate the manipulation of embryonic cells and can be a helpful tool in polyploidy studies and in cytoplasmic transfer experiments.

Citation Download Citation

Annette Clement-Sengewald, Karin Schutze, A. Heinze, G. A. Palma, H. Poesl, and G. Brem "Laser-assisted cell fusion and cytoplast transfer in early mammalian embryos", Proc. SPIE 1876, Lasers in Otolaryngology, Dermatology, and Tissue Welding, (1 July 1993); https://doi.org/10.1117/12.147030

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available