Observation of photodynamically-induced cell destruction probed by video microscopy, laser-scanning microscopy, and fluorescence spectroscopy

Angelika C. Rueck; Wolfgang S. L. Strauss; Michael H. Gschwend; Karsten Koenig; B. Brunner; Herbert Schneckenburger; Heinrich Walt; Rudolf W. Steiner

doi:10.1117/12.147691

7 July 1993 Observation of photodynamically-induced cell destruction probed by video microscopy, laser-scanning microscopy, and fluorescence spectroscopy

Angelika C. Rueck, Wolfgang S. L. Strauss, Michael H. Gschwend, Karsten Koenig, B. Brunner, Herbert Schneckenburger, Heinrich Walt, Rudolf W. Steiner

Author Affiliations +

Proceedings Volume 1882, Laser-Tissue Interaction IV; (1993) https://doi.org/10.1117/12.147691
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

In order to study light-induced reactions during PDT, the fluorescence response of the photosensitizer meso-tetra(4-sulfonatophenyl)porphyrin (TPPS₄) was observed in different cell systems and correlated with the sensitivity to photodynamic induced destructions. RR 1022 epithelial cells from the rat were grown on microscopic slides at a high and low cell density. Using video microscopy in combination with microspectrofluorometry we observed a different fluorescence behavior for high and low cell conditions during light exposure. A fluorescence relocalization from the cytoplasm to the nucleus and an intensity increase-- correlated with the formation of a new molecular species--could be detected only for low cell density. Moreover, cell cultures at a high density showed to be less sensitive to photodynamic destructions. In addition to cell culture-experiments, we observed the light-induced reactions of TPPS₄ accumulated in multicellular tumor spheroids. For these measurements laser scanning microscopy was used. Fluorescence relocalization and intensity increase could be detected only for the peripheric parts of the spheroids. The different fluorescence response seems to reflect different metabolic and physiologic states of the cells.

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Angelika C. Rueck, Wolfgang S. L. Strauss, Michael H. Gschwend, Karsten Koenig, B. Brunner, Herbert Schneckenburger, Heinrich Walt, and Rudolf W. Steiner "Observation of photodynamically-induced cell destruction probed by video microscopy, laser-scanning microscopy, and fluorescence spectroscopy", Proc. SPIE 1882, Laser-Tissue Interaction IV, (7 July 1993); https://doi.org/10.1117/12.147691

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