Detection of single molecules in microspheres

Michael D. Barnes; William B. Whitten; J. Michael Ramsey; Kim C. Ng; Stephen Arnold

doi:10.1117/12.144732

18 May 1993 Detection of single molecules in microspheres

Michael D. Barnes, William B. Whitten, J. Michael Ramsey, Kim C. Ng, Stephen Arnold

Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144732
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

We have investigated the use of micron-sized liquid droplets as sample medium to detect single fluorescent molecules in solution. The use of microdroplets (5 - 15 micrometers diameter) offers several powerful advantages over single-molecule detection schemes involving measurements on bulk liquids where the probe volume is defined by the laser beam. In addition, cavity-quantum electrodynamical (QED) effects have been observed which influence both spontaneous emission rates and fluorescence yields of dye molecules in these microspheres.

Citation Download Citation

Michael D. Barnes, William B. Whitten, J. Michael Ramsey, Kim C. Ng, and Stephen Arnold "Detection of single molecules in microspheres", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144732

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