Near-infrared fluorescent probes

Guillermo A. Casay; Tibor Czuppon; Jacek Lipowski; Gabor Patonay

doi:10.1117/12.144722

18 May 1993 Near-infrared fluorescent probes

Guillermo A. Casay, Tibor Czuppon, Jacek Lipowski, Gabor Patonay

Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144722
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

The application of near-infrared molecules as potential probes for fiber optic applications is discussed. A bis-carboxylic acid derivative of a carbocyanine dye was utilized to determine solution pH changes, and a 2,3-dicyanonaphthalene derivative was used as a probe for the metal concentration studies. The absorbance and fluorescence wavelength of the NIR dyes used in the optical probe are above the 700 nm to minimize interference. The probe dye was entrapped in a polymer matrix attached at the distal end of two optical fibers. The output of a 780 nm semiconductor laser diode beam was focused on one fiber and used for excitation of the probe dye. The second fiber was used to collect the fluorescence from the probe. The fluorescence intensity was monitored as the probe was placed in vials containing the analyte. The optical probe response was found to be about 20 sec during the metal ion measurements; however, the probe response was shorter for pH measurements. The long term stability of the probe was investigated by a week-long period of observation and resulted in a change of about 2% in intensity signal, indicating suitable long term stability.

Citation Download Citation

Guillermo A. Casay, Tibor Czuppon, Jacek Lipowski, and Gabor Patonay "Near-infrared fluorescent probes", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144722

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