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18 May 1993 Phase-locked detection of fluorescence lifetime and its thermometric applications
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Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144713
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States
Abstract
A newly developed category of signal processing scheme for the detection of fluorescence lifetime is discussed, with application to a number of inexpensive, compact, and reliable fluorescence lifetime-based fiber optic sensors. With phase-sensitive detectors being used to achieve a high noise-suppression ability, the schemes discussed here include some of the features of existing phase and modulation techniques for lifetime measurement. However, they differ from them in several aspects, especially in that they operate in a way rather similar to that of a phase-locked loop. Based on such schemes, the measured lifetime is converted to a repetitive signal whose period is directly proportional to it, and thus a high measurement resolution can be achieved. Such schemes can be operated over a wide consecutive measurement range of fluorescence lifetime, although they have been developed for comparably low bandwidth (sub-MHz) use for temperature sensor applications using long lifetime (> 1 microsecond(s) ) fluorescent media. Illustrations of the applications of these schemes in lifetime-based fiber optic thermometers are presented showing their promising potential for both biomedical and industrial uses.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Zhiyi Zhang, Kenneth T. V. Grattan, and Andrew W. Palmer "Phase-locked detection of fluorescence lifetime and its thermometric applications", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144713
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