Ultratrace analysis of biological substances by capillary electrophoresis/semiconductor laser fluorometry

Tetsuhiro Fuchigami; Totaro Imasaka; Masanobu Shiga

doi:10.1117/12.144729

18 May 1993 Ultratrace analysis of biological substances by capillary electrophoresis/semiconductor laser fluorometry

Tetsuhiro Fuchigami, Totaro Imasaka, Masanobu Shiga

Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144729
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

In order to simplify a laser fluorometric detector for capillary electrophoresis a semiconductor laser emitting at 660 nm is used as an exciting source. A labeling reagent fluorescent in the deep-red region is newly synthesized and used for determination of amino acids. The separation resolution is several hundred thousand and the detection limit is subattomole levels. Protein is also labeled by the dye developed and is measured at subfemtomole levels. Contrarily, nonfluorescent samples are detected by indirect fluorometry. Amino acids, flavin adenine dinucleotide (FAD), and deoxyadenosine monophosphate (dAMP) are determined, the detection limit being subpicomole levels.

Citation Download Citation

Tetsuhiro Fuchigami, Totaro Imasaka, and Masanobu Shiga "Ultratrace analysis of biological substances by capillary electrophoresis/semiconductor laser fluorometry", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144729

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