Versatile infrared laser scanner/electrophoresis apparatus

Lyle Richard Middendorf; Robert C. Bruce; Robert D. Eckles; Stephen C. Roemer; Gary D. Sloniker

doi:10.1117/12.144728

18 May 1993 Versatile infrared laser scanner/electrophoresis apparatus

Lyle Richard Middendorf, Robert C. Bruce, Robert D. Eckles, Stephen C. Roemer, Gary D. Sloniker

Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144728
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

An infrared fluorescence microscope consisting of a laser diode for exciting infrared fluorophores attached to DNA oligo-nucleotides and a silicon avalanche photodiode for detecting the infrared emission has been designed. The microscope was mounted on a scanning platform which could be optimally focused on an electrophoretic gel (0.1 - 0.4 mm thick) sandwiched between two glass plates. Background fluorescence is minimal in the infrared region of the optical spectrum. In addition, the optics were designed to further minimize this background fluorescence while maximizing the signal output. A 5 pM fluorophore-DNA concentration in unpolymerized gel solution (about 2000 molecules in an irradiated volume of 600 pL) gave a signal-to-noise ratio of 4:1, 3:1, and 2:1 for a glass-gel-glass sandwich made using quartz, borosilicate, and soda-lime glass, respectively.

Citation Download Citation

Lyle Richard Middendorf, Robert C. Bruce, Robert D. Eckles, Stephen C. Roemer, and Gary D. Sloniker "Versatile infrared laser scanner/electrophoresis apparatus", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144728

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available