Fourier transform infrared analysis of amide III bands of proteins for the secondary structure estimation

Bal Ram Singh; Daniel B. DeOliveira; Fen-Ni Fu; Michael P. Fuller

doi:10.1117/12.145242

1 May 1993 Fourier transform infrared analysis of amide III bands of proteins for the secondary structure estimation

Bal Ram Singh, Daniel B. DeOliveira, Fen-Ni Fu, Michael P. Fuller

Proceedings Volume 1890, Biomolecular Spectroscopy III; (1993) https://doi.org/10.1117/12.145242
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Protein secondary structure has been analyzed using a Fourier transform infrared spectroscopic method in the amide III region. Although extensive work has been done on protein secondary structure using the amide I region (1700 - 1600 cm^-1), the amide III region has not been utilized in the past for its potential in protein structural analysis. One of the major reasons for non-use of the amide III vibrations is perhaps the very weak signal in the amide III frequency region (1200 - 1350 cm^-1). However, benefits of using the amide III region are substantial. For example, water vibrations do not interfere with the protein spectrum unlike in the amide I region. In the amide III region, the protein spectrum is better resolved into individual bands than in the amide I region. This feature allows for a greater ease in peak definement of the protein spectra. In the amide III region, the bands for the individual secondary structures ((alpha) -helix, (beta) -sheet and random coils) do not overlap as much as they do in the amide I region. This lack of overlapping allows for easier and a more reliable means of peak assignment, and secondary structure band positions are easier to determine. Amide III region of protein IR spectra appears to be a valuable tool in estimating the amount of secondary structure present in proteins.

Citation Download Citation

Bal Ram Singh, Daniel B. DeOliveira, Fen-Ni Fu, and Michael P. Fuller "Fourier transform infrared analysis of amide III bands of proteins for the secondary structure estimation", Proc. SPIE 1890, Biomolecular Spectroscopy III, (1 May 1993); https://doi.org/10.1117/12.145242

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