Matrix- and substrate-assisted laser desorption for fast DNA sequencing

C. H. Winston Chen; S. L. Allman; K. Tang; R. B. Jones

doi:10.1117/12.146709

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24 June 1993 Matrix- and substrate-assisted laser desorption for fast DNA sequencing

C. H. Winston Chen, S. L. Allman, K. Tang, R. B. Jones

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Proceedings Volume 1891, Advances in DNA Sequencing Technology; (1993) https://doi.org/10.1117/12.146709
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

During the past few years, tremendous effort has been put to achieving fast sequencing of DNA due to the potential great applications in biological and medical research. Current successful DNA sequencing methods which were developed by Sanger and Maxam-Gilbert have proven very useful for sequencing small DNA segments. However, these methods of sequencing usually involve labeling fragments of DNA for identification following time- consuming gel electrophoresis. The labeling processes usually involve either radioactive tagging or chromophore tagging to various sizes of DNA segments. The segments of DNA are generated either chemically or enzymatically to represent all possible positions of each of the four nucleotides (A, G, C, and T). Both radioactive methods and fluorescent dye labeling methods for DNA sequencing require the use of the time-consuming gel electrophoresis method. It is naturally desirable to consider a time-of-flight mass spectrometer approach to DNA sequencing since the time needed for separation of DNA segments in a mass spectrometer is a few microseconds instead of a few hours in gel electrophoresis.

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C. H. Winston Chen, S. L. Allman, K. Tang, and R. B. Jones "Matrix- and substrate-assisted laser desorption for fast DNA sequencing", Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); https://doi.org/10.1117/12.146709

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