Toward high-resolution imaging of DNA

Fransiska S. Franke; David Keller

doi:10.1117/12.146707

24 June 1993 Toward high-resolution imaging of DNA

Fransiska S. Franke, David Keller

Proceedings Volume 1891, Advances in DNA Sequencing Technology; (1993) https://doi.org/10.1117/12.146707
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Artificial SFM images of DNA calculated from molecular models show that very sharp tips are needed if the internal features of DNA molecules are to be resolved. The calculations are based on 'envelope image analysis', which takes into account only the geometric effect of a tip surface sliding over a sample surface. These calculations show that: (1) A tip of about 5 angstroms end radius of curvature is needed to profile the major groove of double stranded B- form DNA, and an even sharper tip is needed to profile the minor groove. A tip of 1.5 angstroms radius fully profiles the major and minor grooves, and distinguishes the phosphate groups along the backbone. A tip of 20 angstroms end radius shows only a smooth cylinder with no internal detail. (2) it is possible to distinguish various conformations of double- stranded DNA if the backbone spacing is resolved. This seems to require at least a 5 angstroms tip. (3) The individual bases of single-stranded DNA are not easily distinguishable even if the DNA is in a conformation where the bases lie flat on the substrate surface, and even if a very sharp 1.5 angstroms tip is used.

Citation Download Citation

Fransiska S. Franke and David Keller "Toward high-resolution imaging of DNA", Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); https://doi.org/10.1117/12.146707

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available