Principles, practice, and instrumentation for optical recording of cellular metabolism

Barry R. Masters

doi:10.1117/12.146727

24 June 1993 Principles, practice, and instrumentation for optical recording of cellular metabolism

Barry R. Masters

Proceedings Volume 1895, Ultrasensitive Laboratory Diagnostics; (1993) https://doi.org/10.1117/12.146727
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

This chapter describes the principles and practice of the optical recording of cellular metabolism. Redox imaging of cells, tissues, and organs based on intrinsic fluorescent probes of cellular metabolism. Cellular metabolism may be noninvasively interrogated through the `optical method' based on the fluorescence intensity of intrinsic probe molecules (Change, 1991). The intrinsic fluorescent probes, which report on cellular metabolism, are the reduced pyridine nucleotides, NAD(P)H, and the oxidized flavoproteins.

Citation Download Citation

Barry R. Masters "Principles, practice, and instrumentation for optical recording of cellular metabolism", Proc. SPIE 1895, Ultrasensitive Laboratory Diagnostics, (24 June 1993); https://doi.org/10.1117/12.146727

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