Femtosecond excitation transfer processes in biliprotein trimers

A. V. Sharkov; E. V. Khoroshilov; I. V. Kryukov; Lars-Olof Palsson; P. G. Kryukov; R. Fischer; Hella-Christin Scheer; Tomas Gillbro

doi:10.1117/12.146112

17 June 1993 Femtosecond excitation transfer processes in biliprotein trimers

A. V. Sharkov, E. V. Khoroshilov, I. V. Kryukov, Lars-Olof Palsson, P. G. Kryukov, R. Fischer, Hella-Christin Scheer, Tomas Gillbro

Author Affiliations +

Proceedings Volume 1921, Laser Spectroscopy of Biomolecules; (1993) https://doi.org/10.1117/12.146112
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

Femtosecond processes in allophycocyanin, C-phycocyanin and phycoerythrocyanin trimers and monomers have been examined by means of polarization pump-probe technique. No femtosecond kinetics were observed in monomeric preparations. The isotropic absorption recovery kinetics with (tau) equals 440 +/- 50 fs which is not accompanied by anisotropy decay kinetics was obtained in allophycocyanin trimers at 612 nm. The conclusion about energy transfer between neighboring (alpha) 84 and (beta) 84 chromophores with different absorption spectra was made. The proposed model takes into account a stabilizing role of the linker peptide. Spectral and kinetic measurements were made in the 635 - 690 nm spectral region where the proposed acceptor should absorb. The bleaching of the 650-nm band occurs with a delay relative to the bleaching at 615 nm. Only a rise term was observed at 658 nm in consistence with the proposed model. Anisotropy values calculated around 650 nm at 3 ps after excitation are in the range 0.1 - 0.25 corresponding to an angle of 30 degree(s) - 45 degree(s) between the donor and acceptor transition dipole moments. A 500-fs absorption recovery and anisotropy decay process was obtained for C-phycocyanin trimers and explained by Forster energy transfer over 20.8 angstroms between neighboring (alpha) 84 and (beta) 84 chromophores of different monomeric subunits having similar absorption spectra and with a 65 degree(s) angle between their orientations. Energy transfer between violobilin ((alpha) 84) and phycocyanobilin ((beta) 84) chromophores was examined in donor and acceptor spectral regions of phycoerythrocyanin trimers, and was found to take 400 fs.

Citation Download Citation

A. V. Sharkov, E. V. Khoroshilov, I. V. Kryukov, Lars-Olof Palsson, P. G. Kryukov, R. Fischer, Hella-Christin Scheer, and Tomas Gillbro "Femtosecond excitation transfer processes in biliprotein trimers", Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); https://doi.org/10.1117/12.146112

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Chromophores

Absorption

Energy transfer

Polarization

Femtosecond phenomena

Anisotropy

Absorbance

Show All Keywords

Keywords/Phrases

Search In:

Publication Years