Effect of repeated insertions of curved sequences in DNA plasmids: a light-scattering study

Stefano Beretta; Giuseppe Chirico; Giancarlo Baldini; U. Kapp; G. Badaracco

doi:10.1117/12.146215

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17 June 1993 Effect of repeated insertions of curved sequences in DNA plasmids: a light-scattering study

Stefano Beretta, Giuseppe Chirico, Giancarlo Baldini, U. Kapp, G. Badaracco

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Proceedings Volume 1922, Laser Study of Macroscopic Biosystems; (1993) https://doi.org/10.1117/12.146215
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

The effect of the insertion of different amounts (from 0 to 6) of the curved sequence AluI in pUC18m plasmid (2686 base pairs, bp) is studied by dynamic light scattering. This sequence is a highly repeated 113 base pairs long sequence from Artemia Franciscana shrimp. A 30% compaction of the plasmids containing 2 and 6 adjacent AluI sequences compared to pUC8 plasmid (2717 bp) is observed. Furthermore the behavior of the translational diffusion coefficient D_t versus the number of adjacent AluI insertion is not monotonic.

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Stefano Beretta, Giuseppe Chirico, Giancarlo Baldini, U. Kapp, and G. Badaracco "Effect of repeated insertions of curved sequences in DNA plasmids: a light-scattering study", Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); https://doi.org/10.1117/12.146215

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