Molecular self assembly on optical fiber-based fluorescence sensor

Madhu Sudhan Rao Ayyagari; Harry Hong Gao; Bipin Bihari; Kethinni G. Chittibabu; Jayant Kumar; Kenneth A. Marx; David L. Kaplan; Sukant K. Tripathy

doi:10.1117/12.170687

10 March 1994 Molecular self assembly on optical fiber-based fluorescence sensor

Madhu Sudhan Rao Ayyagari, Harry Hong Gao, Bipin Bihari, Kethinni G. Chittibabu, Jayant Kumar, Kenneth A. Marx, David L. Kaplan, Sukant K. Tripathy

Author Affiliations +

Proceedings Volume 2068, Chemical, Biochemical, and Environmental Fiber Sensors V; (1994) https://doi.org/10.1117/12.170687
Event: Optical Tools for Manufacturing and Advanced Automation, 1993, Boston, MA, United States

Abstract

We discuss the molecular self-assembly on optical fibers in which a novel method for protein attachment to the sensing tip of the fiber is used. Our objective is to assemble a conjugated polythiophene copolymer as an attachment vehicle. Subsequent attachment of the photodynamic phycobiliprotein serves as the fluorescence probe element. Following our earlier experiments from Langmuir-Blodgett deposition of these polymeric materials as thin films on glass substrates, we extended the technique to optical fibers. First, the bare fiber surface is silanized with a C18 silane compound. The copolymer (3-undecylthiophene-co-3- methanolthiophene, biotinylated at the methanol moiety) assembly on the fiber is carried out presumable through van der Waals interactions between the hydrophobic fiber surface and the undecyl alkyl chains on the polymer backbone. A conjugated Str-PE (streptavidin covalently attached to phycoerythrin) complex is then attached to the copolymer via the conventional biotin-streptavidin interaction. The conjugated polymer not only supports the protein but, in principle, may help to transduce the signal generated by phycoerythrin to the fiber. Our results from fluorescence intensity measurements proved the efficacy of this system. An improved methodology is also sought to more strongly attach the conjugated copolymer to the fiber surface, and a covalent scheme is developed to polymerize and biotinylate polythiophene in situ on the fiber surface.

Citation Download Citation

Madhu Sudhan Rao Ayyagari, Harry Hong Gao, Bipin Bihari, Kethinni G. Chittibabu, Jayant Kumar, Kenneth A. Marx, David L. Kaplan, and Sukant K. Tripathy "Molecular self assembly on optical fiber-based fluorescence sensor", Proc. SPIE 2068, Chemical, Biochemical, and Environmental Fiber Sensors V, (10 March 1994); https://doi.org/10.1117/12.170687

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